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<t>Human</t> <t>foreskin</t> fibroblast cell lines support the growth of T. pallidum in vitro . A Human foreskin fibroblast cell lines (MoNa, HFF1, and <t>HFFC)</t> support the growth of T. pallidum in long-term, continuous, in vitro cultivation. While cultivation of strain SS14 was discontinued after 10 weeks, in vitro cultures of the DAL-1 strain have been ongoing for over one year. During this period, scheme of subcultures was optimized (up to 1:20). A booster passage (*), extending the standard 7-day subculture to a 14-day period with a cultivation medium exchange on day 7, was employed to enrich the treponemal culture during critical declines. In vitro cultivation was performed in triplicate (i.e., in three cultivation wells) and the mean values for each subculture are presented in graph. Source data from individual wells are shown in Supplementary Table . B Parallel cultivation for seven days ( n = 15) with defined DAL-1 inoculum (10 6 treponemes) validated the differences in growth support of individual foreskin cell lines. Treponemal growth on human foreskin fibroblast cells was slower compared to growth on rabbit epithelial Sf1Ep cells. Red bar, mean. C Human foreskin-based cultivation system supports the growth of various T. pallidum strains ( n = 8), from the Nichols-like as well as the SS14-like cluster. Note that human foreskin fibroblast cells were prepared as an equal mixture of three tested cell lines. Each T. pallidum strain was cultivated in a single in vitro well, representing a sole experimental replicate used for data acquisition

Hffc, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hffc/pmc13037089-41-7-9?v=CLS+Cell+Lines+Service+GmbH
Average 94 stars, based on 9 article reviews

hffc - by Bioz Stars, 2026-08

94/100 stars

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1) Product Images from "First human cell-based cultivation system for the syphilis spirochete Treponema pallidum"

Article Title: First human cell-based cultivation system for the syphilis spirochete Treponema pallidum

Journal: BMC Microbiology

doi: 10.1186/s12866-026-04856-5

Human foreskin fibroblast cell lines support the growth of T. pallidum in vitro . A Human foreskin fibroblast cell lines (MoNa, HFF1, and HFFC) support the growth of T. pallidum in long-term, continuous, in vitro cultivation. While cultivation of strain SS14 was discontinued after 10 weeks, in vitro cultures of the DAL-1 strain have been ongoing for over one year. During this period, scheme of subcultures was optimized (up to 1:20). A booster passage (*), extending the standard 7-day subculture to a 14-day period with a cultivation medium exchange on day 7, was employed to enrich the treponemal culture during critical declines. In vitro cultivation was performed in triplicate (i.e., in three cultivation wells) and the mean values for each subculture are presented in graph. Source data from individual wells are shown in Supplementary Table . B Parallel cultivation for seven days ( n = 15) with defined DAL-1 inoculum (10 6 treponemes) validated the differences in growth support of individual foreskin cell lines. Treponemal growth on human foreskin fibroblast cells was slower compared to growth on rabbit epithelial Sf1Ep cells. Red bar, mean. C Human foreskin-based cultivation system supports the growth of various T. pallidum strains ( n = 8), from the Nichols-like as well as the SS14-like cluster. Note that human foreskin fibroblast cells were prepared as an equal mixture of three tested cell lines. Each T. pallidum strain was cultivated in a single in vitro well, representing a sole experimental replicate used for data acquisition

Figure Legend Snippet: Human foreskin fibroblast cell lines support the growth of T. pallidum in vitro . A Human foreskin fibroblast cell lines (MoNa, HFF1, and HFFC) support the growth of T. pallidum in long-term, continuous, in vitro cultivation. While cultivation of strain SS14 was discontinued after 10 weeks, in vitro cultures of the DAL-1 strain have been ongoing for over one year. During this period, scheme of subcultures was optimized (up to 1:20). A booster passage (*), extending the standard 7-day subculture to a 14-day period with a cultivation medium exchange on day 7, was employed to enrich the treponemal culture during critical declines. In vitro cultivation was performed in triplicate (i.e., in three cultivation wells) and the mean values for each subculture are presented in graph. Source data from individual wells are shown in Supplementary Table . B Parallel cultivation for seven days ( n = 15) with defined DAL-1 inoculum (10 6 treponemes) validated the differences in growth support of individual foreskin cell lines. Treponemal growth on human foreskin fibroblast cells was slower compared to growth on rabbit epithelial Sf1Ep cells. Red bar, mean. C Human foreskin-based cultivation system supports the growth of various T. pallidum strains ( n = 8), from the Nichols-like as well as the SS14-like cluster. Note that human foreskin fibroblast cells were prepared as an equal mixture of three tested cell lines. Each T. pallidum strain was cultivated in a single in vitro well, representing a sole experimental replicate used for data acquisition

Techniques Used: In Vitro

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Journal: BMC Microbiology

Article Title: First human cell-based cultivation system for the syphilis spirochete Treponema pallidum

doi: 10.1186/s12866-026-04856-5

Figure Lengend Snippet: Human foreskin fibroblast cell lines support the growth of T. pallidum in vitro . A Human foreskin fibroblast cell lines (MoNa, HFF1, and HFFC) support the growth of T. pallidum in long-term, continuous, in vitro cultivation. While cultivation of strain SS14 was discontinued after 10 weeks, in vitro cultures of the DAL-1 strain have been ongoing for over one year. During this period, scheme of subcultures was optimized (up to 1:20). A booster passage (*), extending the standard 7-day subculture to a 14-day period with a cultivation medium exchange on day 7, was employed to enrich the treponemal culture during critical declines. In vitro cultivation was performed in triplicate (i.e., in three cultivation wells) and the mean values for each subculture are presented in graph. Source data from individual wells are shown in Supplementary Table . B Parallel cultivation for seven days ( n = 15) with defined DAL-1 inoculum (10 6 treponemes) validated the differences in growth support of individual foreskin cell lines. Treponemal growth on human foreskin fibroblast cells was slower compared to growth on rabbit epithelial Sf1Ep cells. Red bar, mean. C Human foreskin-based cultivation system supports the growth of various T. pallidum strains ( n = 8), from the Nichols-like as well as the SS14-like cluster. Note that human foreskin fibroblast cells were prepared as an equal mixture of three tested cell lines. Each T. pallidum strain was cultivated in a single in vitro well, representing a sole experimental replicate used for data acquisition

Article Snippet: Human foreskin fibroblasts HFF1 (SCRC-1041; ATCC) and HFFC (300715; Cytion) were purchased, while the third cell line (MoNa) was kindly provided by Dr. Vladimir Rotrekl (Masaryk University), and was originally obtained from the National Tissue Centre (Czech Republic).

Techniques: In Vitro

HFFC diet promotes weight gain and MASH. Mice fed control diet or HFFC diet for 24 weeks. ( A , B ) % change in relative body weight and % liver-to-body weight ratio ( A ) and levels of triglyceride and cholesterol in liver ( B ), comparing diets (n = 11–17). ( C ) Liver qPCR for indicated gene expression, normalized by 36b4 (n = 11–15). ( D ) Liver sections were stained with hematoxylin and eosin (H&E) or underwent immunohistochemistry (IHC) with antibody-detecting ki67 or p62, with scale in panel. ( E ) Steatosis and inflammation in liver determined using H&E-stained sections, and % of ki67 positive cells and % area of p62 determined using IHC (n = 8–17). Data in ( A – C , E ) are mean ± standard error of the mean, with individual data points shown. p -value was determined by multiple unpaired t -tests, with adjustment for multiple comparisons. In ( D ), black arrows indicate Ki67 + nuclei and blue arrows indicate p62 + area.

Journal: International Journal of Molecular Sciences

Article Title: Protective Effects of Hepatocyte Stress Defenders, Nrf1 and Nrf2, against MASLD Progression

doi: 10.3390/ijms25158046

Figure Lengend Snippet: HFFC diet promotes weight gain and MASH. Mice fed control diet or HFFC diet for 24 weeks. ( A , B ) % change in relative body weight and % liver-to-body weight ratio ( A ) and levels of triglyceride and cholesterol in liver ( B ), comparing diets (n = 11–17). ( C ) Liver qPCR for indicated gene expression, normalized by 36b4 (n = 11–15). ( D ) Liver sections were stained with hematoxylin and eosin (H&E) or underwent immunohistochemistry (IHC) with antibody-detecting ki67 or p62, with scale in panel. ( E ) Steatosis and inflammation in liver determined using H&E-stained sections, and % of ki67 positive cells and % area of p62 determined using IHC (n = 8–17). Data in ( A – C , E ) are mean ± standard error of the mean, with individual data points shown. p -value was determined by multiple unpaired t -tests, with adjustment for multiple comparisons. In ( D ), black arrows indicate Ki67 + nuclei and blue arrows indicate p62 + area.

Article Snippet: Mice were fed a control diet containing 10% fat (lard and soybean oil), 10% sucrose, 0% fructose, and 0% cholesterol (Research diets, New Brunswick, NJ, USA; catalog# D09100304) or MASH-inducing HFFC diet containing 40% fat (75% palm oil/11% lard/14% soybean oil), 10% sucrose, 20% fructose and 1% or 2% cholesterol (Research diets, New Brunswick, NJ, USA; catalog# D19021910 or D09100310), as indicated in the text and figures.

Techniques: Control, Gene Expression, Staining, Immunohistochemistry

Effect of hepatocyte deficiency for Nrf1, Nrf2, or both in mice chronically fed HFFC diet. Mice fed HFFC diet for 24 weeks. In ( A – C ), mice were infected with indicated virus on week 22. In ( D ), mice were infected on week 16. ( A ) Liver sections stained with hematoxylin and eosin, with scale indicated, and steatosis and inflammation (n = 5–14). ( B ) Levels of triglyceride and cholesterol in liver (n = 6–14). ( C ) qPCR analysis for indicated gene expression, normalized by 36b4 (n = 5–14). ( D ) Representative sections for immunohistochemistry (IHC) with antibody-detecting ki67 or p62, with scale indicated, and % of ki67 positive cells and % area of p62 (n = 6–23). Data are mean ± standard error of the mean, with individual data points shown (males = circles; females = triangle). In ( A – C ), p -value was determined by two-way analysis of variance, with Sidak post-test. In ( D ), p -value was determined by one-way analysis of variance, with Dunnett post-test. In ( D ), black arrows indicate Ki67 + nuclei and blue arrows indicate p62 + area.

Journal: International Journal of Molecular Sciences

Article Title: Protective Effects of Hepatocyte Stress Defenders, Nrf1 and Nrf2, against MASLD Progression

doi: 10.3390/ijms25158046

Figure Lengend Snippet: Effect of hepatocyte deficiency for Nrf1, Nrf2, or both in mice chronically fed HFFC diet. Mice fed HFFC diet for 24 weeks. In ( A – C ), mice were infected with indicated virus on week 22. In ( D ), mice were infected on week 16. ( A ) Liver sections stained with hematoxylin and eosin, with scale indicated, and steatosis and inflammation (n = 5–14). ( B ) Levels of triglyceride and cholesterol in liver (n = 6–14). ( C ) qPCR analysis for indicated gene expression, normalized by 36b4 (n = 5–14). ( D ) Representative sections for immunohistochemistry (IHC) with antibody-detecting ki67 or p62, with scale indicated, and % of ki67 positive cells and % area of p62 (n = 6–23). Data are mean ± standard error of the mean, with individual data points shown (males = circles; females = triangle). In ( A – C ), p -value was determined by two-way analysis of variance, with Sidak post-test. In ( D ), p -value was determined by one-way analysis of variance, with Dunnett post-test. In ( D ), black arrows indicate Ki67 + nuclei and blue arrows indicate p62 + area.

Techniques: Infection, Virus, Staining, Gene Expression, Immunohistochemistry

Effect of hepatocyte deficiency for Nrf1, Nrf2, or both on liver tumor development. ( A ) Study design showing that mice were fed HFFC diet with 2% cholesterol for 52 weeks and infected on weeks 16, 32, and 48 with indicated virus. Liver tumor analysis was performed at the endpoint. ( B , C ) % incidence of liver tumors ( B ) and the number of tumors per liver ( C ) in males (n = 5–7) and females (n = 5–6). ( D ) Volume of largest liver tumor in males (n = 5–7) and females (n = 5–6) and representative tumor images (black arrows indicate tumors). Data are mean ± standard error of the mean, with individual data points shown. The p -value was determined by two-way analysis of variance, with Sidak post-test.

Journal: International Journal of Molecular Sciences

Article Title: Protective Effects of Hepatocyte Stress Defenders, Nrf1 and Nrf2, against MASLD Progression

doi: 10.3390/ijms25158046

Figure Lengend Snippet: Effect of hepatocyte deficiency for Nrf1, Nrf2, or both on liver tumor development. ( A ) Study design showing that mice were fed HFFC diet with 2% cholesterol for 52 weeks and infected on weeks 16, 32, and 48 with indicated virus. Liver tumor analysis was performed at the endpoint. ( B , C ) % incidence of liver tumors ( B ) and the number of tumors per liver ( C ) in males (n = 5–7) and females (n = 5–6). ( D ) Volume of largest liver tumor in males (n = 5–7) and females (n = 5–6) and representative tumor images (black arrows indicate tumors). Data are mean ± standard error of the mean, with individual data points shown. The p -value was determined by two-way analysis of variance, with Sidak post-test.

Techniques: Infection, Virus

Combined effect of Nrf2-inducing drug bardoxolone and Nrf1 overexpression on MASH-linked fibrosis. ( A ) Study design showing C57bl/6J mice that were fed HFFC diet with 2% cholesterol for 24 weeks. Mice were injected with carbon tetrachloride once per week from week 0–15 to induce liver fibrosis. On weeks 16–24, mice were treated as indicated with modulators of Nrf1 and Nrf2 activity. Liver analysis was performed at the endpoint. ( B ) Liver sections stained with hematoxylin and eosin, with scale indicated in panel, and corresponding score for steatosis and inflammation in liver (n = 12–15). ( C ) Liver sections stained with fibrosis detecting sirius red, with scale indicated in panel, and corresponding % sirius red + area (n = 10–14). ( D ) Liver qPCR analysis for indicated gene expression, normalized by ribosomal protein 36b4 (n = 11–15). Data are mean ± standard error of the mean, with individual data points shown (males = circles; females = triangles). The p -value was determined by one-way analysis of variance, with Dunnett post-test.

Journal: International Journal of Molecular Sciences

Article Title: Protective Effects of Hepatocyte Stress Defenders, Nrf1 and Nrf2, against MASLD Progression

doi: 10.3390/ijms25158046

Figure Lengend Snippet: Combined effect of Nrf2-inducing drug bardoxolone and Nrf1 overexpression on MASH-linked fibrosis. ( A ) Study design showing C57bl/6J mice that were fed HFFC diet with 2% cholesterol for 24 weeks. Mice were injected with carbon tetrachloride once per week from week 0–15 to induce liver fibrosis. On weeks 16–24, mice were treated as indicated with modulators of Nrf1 and Nrf2 activity. Liver analysis was performed at the endpoint. ( B ) Liver sections stained with hematoxylin and eosin, with scale indicated in panel, and corresponding score for steatosis and inflammation in liver (n = 12–15). ( C ) Liver sections stained with fibrosis detecting sirius red, with scale indicated in panel, and corresponding % sirius red + area (n = 10–14). ( D ) Liver qPCR analysis for indicated gene expression, normalized by ribosomal protein 36b4 (n = 11–15). Data are mean ± standard error of the mean, with individual data points shown (males = circles; females = triangles). The p -value was determined by one-way analysis of variance, with Dunnett post-test.

Techniques: Over Expression, Injection, Activity Assay, Staining, Gene Expression

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